cht ii hydroxyapatite resin  (Bio-Rad)

Journal: Protein expression and purification

Article Title: A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process.

doi: 10.1016/j.pep.2023.106241

Figure Lengend Snippet: Fig. 5. Milligram Scale CHT Chromatography and Corresponding HPSEC Profile for SARS-CoV-2 Spike Protein RBD. 5A shows the chromatography profile of SARS- CoV-2 Spike Protein RBD from the CHT resin. Flow through, wash, and strip peaks were observed. 5B, 5C, 5D, and 5E respectively show the flow through, wash 1, wash 2, and strip HPSEC purity profiles. UV A280, conductivity, and pH traces are respectively shown in blue, orange, and purple.

Article Snippet: Development of a scalable aggregate removal operation to replace the SEC operation consisted of studying the behavior of SARS-CoV-2 Spike Protein RBD from the Wuhan strain on CHT Type II resin (BIORAD; Hercules, CA, USA) resin (1.6 cm column diameter, 14.8 cm bed height).

Techniques: Chromatography, Stripping Membranes

Journal: Protein expression and purification

Article Title: A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process.

doi: 10.1016/j.pep.2023.106241

Figure Lengend Snippet: Fig. 8. SDS-PAGE and Western blot Analyses of Gram Scale SARS-CoV-2 Spike Protein RBD. The CHT Type II gram scale product was analyzed by SDS-PAGE in 8A and Western blot in 8B. The milligram control and CHT Flow Through Products were visualized by reaction with a biotinylated polyclonal antibody specific for the poly-histidine tag prior to application of a streptavidin labeled peroxidase. Extraneous lanes were removed from the image.

Article Snippet: Development of a scalable aggregate removal operation to replace the SEC operation consisted of studying the behavior of SARS-CoV-2 Spike Protein RBD from the Wuhan strain on CHT Type II resin (BIORAD; Hercules, CA, USA) resin (1.6 cm column diameter, 14.8 cm bed height).

Techniques: SDS Page, Western Blot, Control, Labeling

Journal: Protein expression and purification

Article Title: A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process.

doi: 10.1016/j.pep.2023.106241

Figure Lengend Snippet: Fig. 7. Gram Scale CHT Elution and Corresponding HPSEC Profile for SARS-CoV-2 Spike Protein RBD. 7A shows the elution profile of SARS-CoV-2 Spike Protein RBD from CHT Type II resin. 7B, 7C, and 7D contain the respective HPSEC purity profiles of the flow through, re-equilibration (Wash 1), and high phosphate (Wash 2) CHT products. The UV A280, conductivity, and pH traces are respectively shown in blue, orange, and purple.

Article Snippet: Development of a scalable aggregate removal operation to replace the SEC operation consisted of studying the behavior of SARS-CoV-2 Spike Protein RBD from the Wuhan strain on CHT Type II resin (BIORAD; Hercules, CA, USA) resin (1.6 cm column diameter, 14.8 cm bed height).

Techniques:

Journal: Protein expression and purification

Article Title: A scalable and high yielding SARS-CoV-2 spike protein receptor binding domain production process.

doi: 10.1016/j.pep.2023.106241

Figure Lengend Snippet: Fig. 11. CHT Elution and Corresponding Purity Profiles of SARS-CoV-2 Omicron BA.2 Spike Protein RBD. 11A shows the elution profile of SARS-CoV-2 Omicron Spike Protein RBD from CHT Type II resin. 11B, 11C, 11D, and 11E contain the respective HPSEC purity profiles of the flow through, re-equilibration (Wash 1), and high salt (Strip1) and high phosphate (Strip 2) CHT products. The UV A280, conductivity, and pH traces are respectively shown in blue, orange, and purple. 11F shows the SDS-PAGE analysis of the monomeric flow through product.

Article Snippet: Development of a scalable aggregate removal operation to replace the SEC operation consisted of studying the behavior of SARS-CoV-2 Spike Protein RBD from the Wuhan strain on CHT Type II resin (BIORAD; Hercules, CA, USA) resin (1.6 cm column diameter, 14.8 cm bed height).

Techniques: Stripping Membranes, SDS Page